![]() ![]() The study indicated that cocaine combined with any of these substances showed greater toxicity than that observed with single components. Atropine is an important antidote when one is poisoned with a cholinesterase-inhibiting insecticide. The symptoms are therefore the opposite of those caused by muscarine or acetylcholine ( pupil dilation, dry mouth, inhibition of sweating, tachycardia, palpitations, hallucinations, delirium, etc.). This toxicant also binds specifically to the muscarinic receptors where it blocks the effect of ACh. Of direct relevance for pesticide science is the antagonist atropine. Development and validation of an LC/MS/MS method for the determination of L-hyoscyamine in human plasma. Mobile phase MeCN MeOH 10 mM ammonium acetate 62.5 37.5 15 ĭetector MS, Perkin Elmer Sciex API Ill-Plus triple quadrupole, APCI, nebulizer 400° and 80 psi, auxiliary nitrogen 1.2 L/min, curtain gas 1.2 L/min, interface 55°, collision gas argon, electron multiplier 3000 V, declustering potential 35 V, collision energy 35 eV Remove the organic layer and evaporate it to dryness under a stream of nitrogen at 40°, reconstitute the residue in 100 xL mobile phase, inject a 20 pL aliquot. Sample preparation 1 mL Plasma + 100 p,L 5 ng/mL scopolamine in MeOH, vortex briefly, add 50 jaL 1 M ammonium hydroxide, mix, add 5 mL dichloromethane, shake horizontally for 5 min, centrifuge at 2500 rpm for 5 min. 51-55-8 (atropine), 52-88-0 (atropine methyinitrate), 101-31-5 (hyoscyamine), 306-03-6 ( hyoscyamine hydrobromide), (hyoscyamine suifate dihydrate), 620-61-1 (hyoscyamine suifate)
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